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polyclonal goat anti-human fh ab  (EuroClone)

 
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    Structured Review

    EuroClone polyclonal goat anti-human fh ab
    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
    Polyclonal Goat Anti Human Fh Ab, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal goat anti-human fh ab/product/EuroClone
    Average 90 stars, based on 1 article reviews
    polyclonal goat anti-human fh ab - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Complement Factor H Family Proteins Modulate Monocyte and Neutrophil Granulocyte Functions"

    Article Title: Complement Factor H Family Proteins Modulate Monocyte and Neutrophil Granulocyte Functions

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.660852

    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using polyclonal anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
    Figure Legend Snippet: FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using polyclonal anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.

    Techniques Used: Binding Assay, Flow Cytometry, Incubation



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    polyclonal goat anti-human fh ab  (EuroClone)
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    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
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    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
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    polyclonal goat anti-human fh ab  (Millipore)
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    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using <t>polyclonal</t> anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.
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    Image Search Results


    FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using polyclonal anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.

    Journal: Frontiers in Immunology

    Article Title: Complement Factor H Family Proteins Modulate Monocyte and Neutrophil Granulocyte Functions

    doi: 10.3389/fimmu.2021.660852

    Figure Lengend Snippet: FHR-1, FHR-5, FHL-1 and mini-FH bind to primary neutrophil granulocytes and monocytes. Binding of 300 nM of each protein (solid lines) to neutrophil granulocytes and monocytes in Ca 2+ and Mg 2+ containing DPBS was measured by flow cytometry. Filled histograms indicate autofluorescence, dotted lines show samples incubated without the proteins added (antibody controls). Binding of the added proteins (solid black line) was analyzed using polyclonal anti-FH Ab for FHR-1, FHL-1 and mini-FH detection, and monoclonal anti-FHR-5 for FHR-5 detection. A representative histogram out of three independent experiments is shown in each case.

    Article Snippet: Bound proteins were detected via incubation with the polyclonal goat anti-human FH Ab in DPBS containing fetal bovine serum (FBS, 0.1%, EuroClone, Pero, Italy) for 15 minutes at 4°C, washed and labelled with Alexa-488-conjugated rabbit anti-goat antibody (Thermo Scientific, Waltham, MA) under the same conditions.

    Techniques: Binding Assay, Flow Cytometry, Incubation